Effect of bo
Effect of bone marrow-derived mesenchymal stem cell transplantation on apoptosis and expression of P75NTR, TrkA in rats with intracerebral hemorrhage
Yingke Yang1, Bo Song1, Guangming Gong2, Yanlin Wang1, Jing Qi1,Huili Zhang1, Yuming Xu1, Hongcan Zhu1
1. The Third Department of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P. R. China
2. Department of Microbiology and Immunology, College of Basic Medicine, Zhengzhou University, Zhengzhou, Henan 450001, P. R. China
Abstract: Intracerebral hemorrhage (ICH)is a kind of serious nervous system disease of high incidence, morbidity and mortality. The major problem is that it leaves severe neurologic dysfunction even after recovery,threatens human health and lacks of effective treatment. Many researches on bone marrow-derived mesenchymal stem cells(BMSCs) transplantation have affirmed the improve effect on the recovery of the neurologic dysfunction of ICH,but the mechanism is still in exploration.So a rat model of ICH is established by stereotaxical injection of collagenase Ⅶ into the striatum and BMSCs are injected around the hematoma.We evaluate the change of neurologic function on day 1, 3, 7, 14 and 28 after ICH surgery by modified neurological severity score (mNSS) table and detect the change of expression of p75 neurotrophin receptor(P75NTR),tyrosine kinase A receptor(TrkA) and the cell apoptosis around the hematoma.It is found that the neurological scores of rats are obviously improved and the apoptotic cells are significantly reduced with lower P75NTR and higher TrkA expression compared with the ICH group. Our research shows thatBMSCs transplantation could significantly promote the neurological function recovery in a rat model of ICH, which might be associated with its role in anti-apoptotic by down-regulating the expression of P75NTR and up-regulating the expression of TrkA in tissue around hematoma.
[Yang YK, Song B, Gong GM, Wang YL, Qi J, Zhang HL, Xu YM, Zhu HC. Effect of bone marrow-derived mesenchymal stem cell transplantation on apoptosis and expression of P75NTR, TrkA in rats with intracerebral hemorrhage. Life Sci J 2013;10(1):2749-2753] (ISSN:1097-8135). http://www.lifesciencesite.com.
Key words：bone marrow-derived mesenchymal stem cells (BMSCs), intracerebral hemorrhage (ICH), apoptosis, p75 neurotrophin receptor (P75NTR), tyrosine kinase A receptor (TrkA).
