Augmenting An
Augmenting
Anticancer Potential of Exotoxin A By Mutating Pseudomonas aeruginosa.
Yehia A. O. Ellazeik¹, Samah
S. Abdelgawad², Essam MA Elsawy², Ahmed M. El-Wassef3.
¹Department of Botany,
Faculty of Science, Mansoura University.
²Microbiology lab, Urology
and Nephrology Center, Mansoura University.
3Department of Chemistry, Faculty of Science, Mansoura
University.
Abstract: Cancer
is a group of more than 100 different and distinctive types of diseases. It is
an abnormal growth of cells which tend to proliferate in an uncontrolled manner
forming masses and in some cases dislodge and spread all over the body.
Luckily, a number of bacteria including Pseudomonas aeruginosa produce
some virulence factors that help in combating cancer such as exotoxin A
(ETA). This toxin arrests protein synthesis and induces apoptosis in
cancer cells. During this study 68 Pseudomonades were isolated from urine
samples collected from patients with urinary tract infection during the period
Sept. 2009- Feb. 2010. Classical bacteriological, molecular and automated
methods were used to identify all them. Based on the protein banding patterns,
the 68 isolates were re-grouped to 30according to the results of Sodium
dedocylsulfate polyacrylamide gel electrophoresis (SDS-PAGE), from these 30
isolates only three gave the typical PCR product for Exo A gene using its
specific primers. ETA production and toxicity were enhanced by mutating the
three wild-type isolates. The crude and partially purified ETA from the
selected three wild-type isolates of Pseudomonas aeroginosa
(1, 8, 15) and two UV mutants (3-1 and 16-15) showed a promising inhibitory
activity against the MCF-7 cell line of breast carcinoma. The IC50 (inhibition
concentration) of the five organisms were 14.1, 35.6, 36.8, 5.3, 3.4µg,
respectively. The mutation increased the anticancer activity of ETA
2-fold for one mutant and 10-fold to the second mutant. No change in the
molecular weight of the mutated protein was found and the exact nature of its
anticancer activity is under further investigation.
[Yehia A. Ellazeik, Samah S. Abdelgawad,Essam MA
Elsawy and Ahmed M. El-Wassef. Augmenting Anticancer Potential of
Exotoxin A By Mutating Pseudomonas aeruginosa. J Am Sci 2013;9(3):312-321].
(ISSN: 1545-1003). http://www.jofamericanscience.org. 55
Key Words: Pseudomonas aeruginosa;virulence factors;cancer;Exotoxin A(ETA);PCR. Full Text 55
