Detection of C
Detection
of Circulating Microparticles in Patients with Proliferative Diabetic
Retinopathy
Samy A Khodeir 1,
Y M Abd El Raouf 1, Gihan Farouk2 and Mohammed
EL-Bradey3
Departments of 1Internal
Medicine 2Clinical Pathology and 3Ophthalmology,
Faculty of Medicine, Tanta University.[email protected]
Abstract: Background: The development of vasculopathies in
diabetes involves multifactorial processes including pathological activation of
vascular cells. Release of microparticles by activated cells has been reported
in diseases associated with thrombotic risk, but few data are available in
diabetes. Diabetic retinopathy is associated with increased local activation or
apoptosis of retinal, neural, and vascular `endothelial cells in the eye which
indicate that microparticles (MPs) of different cellular origin might be
locally generated in the eye of diabetic patients. Aim: The aim of this study
is to investigate the presence of endothelial, platelet, and retinal-derived
microparticles both in the vitreous and in the plasma of diabetic patients
compared with that of non diabetic ones. Subjects and methods: In a
case-control study, this study included 45 patients: 25 diabetic patients with
non proliferative diabetic retinopathy (NPDR), and 20 diabetic patients with
proliferative diabetic retinopathy (PDR) compared with control group consists
of non diabetic 10 subjects. Blood samples were analyzed by flow cytometry.
Microparticles present in plasma and vitreous were analyzed according to their
parameters of size and fluorescence. Results: As regard plasma samples, there
was significant increase in CD144 and CD41 in groups II, III as compared with
control group (p = 0.001). Peanut agglutinin PNA was not detected in plasma
sample among all studied groups. Whereas, as regard to the vitreous sample,
there was significant increase in CD144, CD41 and PNA in groups II,III as
compared with control group (p= 0.048, 0.009, 0.048 ), (P = 0.001)
respectively. Conclusion: microparticles appear as a new prognostic potential of
type 2 diabetes in the early detection of vascular complications.
Moreover significant increase of different types of microparticles
in vitreous fluid of membrane in patients with PDR, may contribute to disease
progression.
[Samy A Khodeir , Y M Abd El Raouf , Gihan
Farouk and Mohammed EL-Bradey. Detection of Circulating Microparticles
in Patients with Proliferative Diabetic Retinopathy. Life Sci J
2012;9(1):204-209] (ISSN: 1097-8135).http://www.lifesciencesite.com. 29
Key
words: Microparticles,
diabetic retinopathy, endothelial cell dysfunction. Full Text 29
