Hepato-protect
Hepato-protective
Effect of Green Tea Extract on Cyclosporine a Treated Rabbits: Histological and
Ultrastructural Study
Fathy Ahmed Fetouh and Amal
Al Shahat
Department of Anatomy,
Faculty of Medicine, Zagazig University
Abstract: Cyclosporine
A (CsA) has a biologic activities, including anti-parasitic, fungicidal
and anti-inflammatory effects by inhibiting T-cell activation. CsA and its
metabolites are taken and excreted by the hepatocytes into the bile and a small
portion into the blood. Green tea contains several antioxidants and has a role
as an antioxidant. Objective: The present work aimed to study
the structural hepato-protective effect of green tea extract on rabbits treated
with cyclosporine A (CsA). Materials and methods: Three groups
of adult rabbits were used for this study; the 1st group was
used as a control, the 2nd group was treated with cyclosporine
A (CsA) as oral solution in a dose of 15 mg/kg for 4 weeks and the 3rd group
was treated with cyclosporine A in the same dose simultaneously with green tea
extract for the same period. The animals were anaesthetized and liver specimens
were obtained and semi-thin sections of 1 mm thick were obtained, stained
with 1% toluidine blue and examined by light microscopy and ultrathin sections
for electron microscopic examination. Results: The
cyclosporine A had adverse effects on the liver cells of cyclosporine A-treated
animals in comparison with that of the control group where, the hepatocytes
showed extensive vacuolization and variable areas of fatty degeneration. Also,
the cytoplasm showed reduced organelles, as few mitochondria with loss of their
transverse cristae, few glycogen particles and loss of endoplasmic reticulum.
In some cells the cytoplasm showed loss of the organelles and depletion of
glycogen content. The nucleus appeared irregular with deformed nuclear
membrane. A concomitant administration of green tea extract to cyclosporine A treated
rabbits markedly prevented the adverse effects of cyclosporine A where the
hepatocytes preserved their normal appearance. The cytoplasm showed numerous
mitochondria with well developed transverse cristae, smooth and rough
endoplasmic reticulum, Golgi apparatus and abundant amount of glycogen
particles. The nucleus appeared round and light with well formed nuclear
membrane. Also, bile canaliculi and the microvilli projecting from the cells
were preserved. Conclusion: The green tea extract had
beneficial effects against cyclosporine A induced hepatic toxicity and should
be used in combination with cyclosporine A transplant treatment to improve the
cyclosporine A induced oxidative stress parameters and other adverse effects.
[Fathy Ahmed Fetouh and Amal Al Shahat. Hepato-protective
Effect of Green Tea Extract on Cyclosporine a Treated Rabbits: Histological and
Ultrastructural Study. Life Sci J 2013;10(1):1924-1932]
(ISSN:1097-8135).http://www.lifesciencesite.com.
Key words: Green tea extract, Cyclosporine A, Hepatocytes, Ultrastructure.
