Silymarin Amel
Silymarin
Ameliorates Cisplatin-Induced Hepatotoxicity in Male Rabbits
Gamal H. Abdel-Rahman1 and
El-Sayed K. Abdel-Hady2
Current address: 1,2 Biology Department, Faculty
of Science, Taif University, Taif, KSA
1Zoology Department, Faculty of Science, Assiut
University, Assiut, Egypt
2Zoology Department, Faculty of Science, Mansoura
University, Mansoura, Egypt
Abstract: Cisplatin
(CDDP) is a widely used anticancer drug, but at high dose, it can produce
undesirable side effects such as hepatotoxicity. In this study, the protective
activity of silymarin against hepatic tissue damage induced by repeated
administration of cisplatin was analyzed morphologically and biochemically.
Male Newzeland rabbits were divided into four groups, 6 rabbits in each.
Control group, silymarin group (100 mg /kg b.wt./day), Cisplatin treated
group (3.5 mg /kg b. wt./day) and Cisplatin plus silymarin treated
group. Results revealed that cisplatin caused histopathological effects on
the hepatic tissue. These effects includes vacuolation of cells, lymphocytic
infiltration, dilation of blood sinusoids and haemorrhage. Histochemical observations revealed a
marked depletion of polysaccharide and proteins in the liver cells of cisplatin
treated animals. Cisplatin hepatotoxicity was manifested biochemically by
elevation of MDA and decrease in GSH and the activities of SOD in the liver
tissues. Results showed that administration of cisplatin increased the
immunohistochemical expression of Bcl-2 protein. Treatment with silymarin
reduced histopathological and biochemical alterations in the liver tissue. These
results suggested that silymarin possess protective effects against cisplatin
hepatotoxicity in rabbits.
[Gamal H. Abdel-Rahman and El-Sayed K. Abdel-Hady. Silymarin
Ameliorates Cisplatin-Induced Hepatotoxicity in Male Rabbits. Life
Sci J 2013;10(1):3333-3341]. (ISSN: 1097-8135).http://www.lifesciencesite.com.
Key words: Cisplatin - Silymarin – Rabbits – Histopathology – Lipid peroxidation – Immunohistochemistry.
