The use of mic
The use of microarrays to reveal the probabilistic gene network associated with the response of rice to low-energy ion beam bombardment
Xiangli Yu, Yonghui Li and Huiyuan Ya*
Life Science Department, Luoyang Normal University, Luoyang, Henan, China
Abstract：Bombardment with low-energy ion beams can induce various biological effects in plants including stimulation, damage, and mutation. However, the interactions in genes in plants in response to this stressor are not fully understood. We use a Rice Gene Expression Microarray to investigate the overlap differentially expressed genes (DEGs) in two independent bombardment experiments involving three ion fluences. The results show that 26 up- and 6 down-regulated overlap genes were observed. A RiceNet co-expressed network analysis of the overlap DEGs showed the direct and indirect co-expressed linkages, which suggests some signal transduction pathways should be involved in this response but the situation remains unclear. Our microarray data reveals the general and key genes responding to the stress of bombardment with low-energy ion beams during rice germination, and provides information on the candidate genes for further elucidation of the molecular mechanisms biological effects underlying the of ion-beam bombardment.
[Xiangli Yu, Yonghui Li and Huiyuan Ya. The use of microarrays to reveal the probabilistic gene network associated with the response of rice to low-energy ion beam bombardment. Life Sci J 2013;10(1):3066-3069] (ISSN:1097-8135). http://www.lifesciencesite.com. 9
Key words：Low-energy ion beam bombardment; biological effect; microarray; gene network; rice. l
