Serum Antibody D
Serum Antibody Detection in Ecchinococcosis: Specificity
of Hydatidosis enzyme-linked immunosorbent assay (ELISA) IgG
Metwally D M1&2 and
Al-Olayan E M2
1Parasitology Department, Faculty of Veterinary
Medicine, Zagazig University, Zagazig, Egypt.
2Zoology Department ,Faculty of Science ,King Saud
University, Riyadh, KSA.
[email protected];
[email protected] ksu.edu.sa
Abstract: Hydatidosis
is diagnosed by serological together with radiological and ultrasound
examinations .but cross -reactivity with other parasites, over evaluating the
real prevalence, represents a big limitation. The aim of the present study was
to develop a specific and simple antibody detection (IgG) -based ELISA
method .The samples in this study included 35 patients in the following
groups: IHAT(Indirect Haem Agglutination Test) and abdominal ultrasonography confirmed
hydatidosis patients (20 cases), control with other parasitic diseases (5cases
Toxoplasmosis) and healthy controls (10cases). Antibody detection by indirect
ELISA usingEcchinococcus granulosus antigen showed that 65% of
hydatic patients (13cases) have anti-hydatid cyst antibodies in their serum
while no cross reaction was detected. A sensitivity of 72.22% and specificity
of 75% were found for the antibody detection assay. Findings of this study
indicated that antibody detection assay is a highly sensitive and specific
approach for diagnosis of hydatid cyst.
[Metwally D M and Al-Olayan E M. Serum
Antibody Detection in Ecchinococcosis: Specificity of Hydatidosisenzyme-linked
immunosorbent assay (ELISA) IgG. Life Sci J 2013;10(1):2107-2110].
(ISSN: 1097-8135).http://www.lifesciencesite.com.
Keywords: hydatidosis, ELISA IgG diagnosis, sensitivity, specificity.
