Bacterial Contami
Bacterial Contaminating the Haemodialysis
Dialysate-Water Exhaust–Origin and Fate at Al-Madinah Al-Mounwwarah
Atef
M. Diab1 and Idriss Mouneer Al-Turk 2
1Faculty of Applied Medical Sciences, Al-Madinah Al-
Mounwwarah, Kingdom of Saudi Arabia.
2 Faculty of Science, Taibah University,
Al-Madinah Al- Mounwwarah, Kingdom of Saudi Arabia.
Corresponding author: i[email protected]
ABSTRACT: Qualitative
and quantitative bacteriological analysis of the dialysate-water exhaust for
the only two haemodialysis units found at Al-Madinah Al-Mounwwarah, were
studied. Ten samples were collected over 75 days, 5 times from each site of
sampling on biweekly basis. Enumeration of total viable (TVB) and total
coliform (TC) bacterial counts CFU/ ml, showed figures ranged from 0.0 to
5231x102 CFU /ml and from 0.0 to 73x102 CFU
/ml, respectively. Specific identification of the isolated bacterial
strains using API 20 E strips confirmed that isolated bacterial
populations composed of 6 genera in one unit and 7 in the other. Bacterial
diversity was relatively poor, but the counts were obviously-high.Determination
of MIC(s), MBC(s) ,as well as, the MIC/MBC indexes
for 153 representative strains of the bacterial population isolated in the
study were done. All the studied strains exhibited resistance to at least 3,
out of the 9 tested antibiotics up to MIC(S) > 100 μg/ml.
[Atef
M. Diab and Idriss Mouneer Al-Turk. Bacterial Contaminating the
Haemodialysis Dialysate-Water Exhaust–Origin and Fate at Al-Madinah
Al-Mounwwarah. Life Sci J 2013;10(1):1030-1035]
(ISSN:1097-8135).http://www.lifesciencesite.com.
Key Words: Haemodialysis, Gram–ve Bacteria of Dialysate Exhaust, Sheering effect, MIC/MBC index
