Colchicine Induct
Colchicine Induction of Polyploidy in
Egyptian Clover Genotypes
Abd El-Naby, Zeinab, M1.*,
Nabila A. Mohamed1, Khalid H. Radwan2, andDina A.
El-Khishin2
1Field Crops Research Institute (FCRI). Agricultural
Research Center (ARC) 9, Gamma St., Giza-12619, Giza, Egypt; 2Agricultural
Genetic Engineering Research Institute (AGERI). Agricultural Research Center
(ARC) 9, Gamma St., Giza-12619, Giza, Egypt. [email protected]
Abstract:
Induction of polyploidy and chromosome comparison of eight berseem clover (Trifolium
alexandrinum L.) genotypes were performed. Different
colchicine concentrations were applied to seeds and seedlings using two
methods: immersion and sprinkling. Both methods were successful in polyploidy
induction. Karyotyping of F1 seeds andtheir parents were examined
for polyploidy. Chromosome number was determined in root-tip cells 2-3d old
from seed sowing. Tetraploids were evident in all populations (2n= 4X=32),
whereas diploid parents had (2n=2X=16) chromosomes. Interactions between
genotypes and colchicine treatments recorded high significant differences of
no. inflorescences plant-1, 1000-seed weight, no. floret
inflorescence-1 and no. seeds floret-1 with
significant differences of seed weight g m-2, seed setting% and
mortality%. Higher variation in leaflet shape and number, inflorescence size
and shape and floret size was viewed over all colchicine treatments in
tetraploid plants compared with the control. High positive significant
correlation coefficient between number of seeds per floret and number of florets
plant-1 (r=0.853**) and seed setting% (r=0.943**), mortality%
was negatively correlated with seed setting% (-0.795**). Positive significant
correlation was found between 1000-seed weight and number of florets plant-1
(r=0.711*) and seed setting% (r=0.744*).
Polyploidy induction resulted in taller plants, faster re-growth after cutting,
increased tillering and branching plant-1, increases in 1000 seed
weight and increased seed yield m-2. This methodology can be used in
plant breeding programs to increase forage production efficiency and select for
more superior adaptive genotypes.
[Abd El-Naby, Zeinab, M, Nabila A. Mohamed, Khalid H.
Radwan, and Dina A. El-Khishin. Colchicine Induction of Polyploidy in
Egyptian Clover Genotypes. J Am Sci 2012;8(10):221-227].(ISSN:
1545-1003).http://www.jofamericanscience.org. 33
Key words: Berseem
clover, Colchicine, Chromosomes, Correlation, Ploidy. Full Text 33
