Effect of GLP-1 o
Effect of GLP-1 on after Experimental
Ischemic Reperfusion Injury in Rats
Hany El Sebaee1;
Maged Haroum1 and Rehab Ahmed Mohammed1 Ahmed
Soliman2
Departments of 1Physiolog
and 2Pathology, Faculty of Medicine, Kasr Al-Aini Cairo
University Egypt.
Abstract: Glucagon-like
peptide-1 (GLP-1) is an incretin hormone secreted by L-cells of small intestine
in response to nutrient ingestion. Although the major physiological function of
GLP-1 appears to be in relation to glycaemic control, there is growing evidence
to suggest that it may also play an important role in the cardiovascular
system. GLP-1 receptors (GLP-1Rs) are expressed in the heart and vasculature of
both rodents and humans, and recent studies have demonstrated that GLP-1R
agonists have wide-ranging cardiovascular actions, such as modulation of heart
rate, blood pressure, vascular tone and myocardial contractility. In this
study the cardiac effect of native GLP-1 after experimental induction of
ischemia was studied. Fifty rats were used in this study. Their weight
ranged from 200-250 grams. Rats were
anesthetized and the hearts were
excised. The hearts were mounted on a Langendorff perfusion system and a
retrograde perfusion was started within 3min of the heart excision.
After 30 min of stabilization, the following
groups were defined: (1) Control
group (group1a): consists of 10 rats, no ischemia, the flow was
continuous for 2 hours. (Sham operation). In the other groups the flow was turned off for 35 min to elicit global
ischemia and reperfusion was continues for 120min. (2) Control
group (group1b): consists of 10 rats,
no pharmacological agents were added during the first 15min of reperfusion. (3) DPP4
inhibitor group (group2): consists of 10 rats, sitagliptin 20mg/l was added during the first 15min of
reperfusion. (4) GLP-1group (group3): consists
of 10 rats, GLP-1 (0.3nM/l) + sitagliptin 20mg/l were
added during the first 15min of reperfusion. (5) GLP-1
high dose group (group4):consists of 10 rats,
GLP-1(10.3nM) + sitagliptin 20mg/l were added during the first 15min of
reperfusion. The following parameters were measured at the
end of stabilization period (reading1) and at the end of reperfusion (reading2) 1- Heart rate / min. 2-Left ventricular developed
pressure/mmHg =left ventricular systolic-diastolic pressure. 3-Rate pressure
product/mmHg/min =HR × LVDP and
4-Maximum rate of pressure rise ΔP/ΔT /mmHg/sec. Histopathological studies of sections from the 5 studied
groups were performed usingHematoxylin and Eosin.
[Hany El Sebaee; Maged Haroum; Rehab Ahmed Mohammed
and Ahmed Soliman. Effect of GLP-1 on after Experimental Ischemic
Reperfusion Injury in Rats. Life Sci J 2013;10(1):3426-3437].
(ISSN: 1097-8135).http://www.lifesciencesite.com.
Keywords: GLP-1, Sitagliptin, Mycaardial Infarction, Reperfusion Injury.
