Prevalence of Pla
Prevalence of Plasmodium Vivax Using PCR Method in Afghan Refugee Ghamkol Camp District Kohat, Pakistan
Sultan Ayaz 1, Riaz Ullah 2, Shehzad Zarin 1
1Department of Zoology, Kohat University of Science and Technology Kohat, KPK, 26000, Pakistan
2Department of Chemistry, Sarhad University of Science and Information Technology, Peshawar, KPK, Pakistan
Corresponding Author Dr Riaz Ullah. [email protected]
Abstract: Malaria is a disease caused by protozoan parasites belongs to the genus Plasmodium, which is transmitted by female Anopheles mosquito. It causes more than one million deaths per year. Afghan Refugees in Pakistan are at higher risk of malarial infection. Blood samples were collected from Afghan refugees in Ghamkol camp district Kohat. The samples were analyzed by Hemometer and Polymerase Chain Reaction. A total of 220 blood samples were examined by poly merease chain reaction (PCR) from susceptible peoples resides in Afghan refugee Ghamkol camp district Kohat. The DNA were extracted and amplified through PCR which confirmed the P.vivax detection. Overall prevalence of malaria 50.00% (110/220), among these Males were 50.00% (78/156) and females were 46.87% (30/64). It was observed an average low level of Hb(9.98gm/dl) in P. vivax infection patients. It was concluded that children at the age 1-20 years were more susceptible for malarial infection than above ages. Plasmodium vivax was more prevalent in Afghan refugee Ghamkol camp Kohat.
[Sultan Ayaz, Riaz Ullah, Shehzad Zarin. Prevalence of Plasmodium Vivax Using PCR Method in Afghan Refugee Ghamkol Camp District Kohat, Pakistan Life Sci J 2013;10(1):2948-2953] (ISSN:1097-8135).http://www.lifesciencesite.com.
Keywords: Prevalence, Plasmodium vivax and PCR.
