Synergism Effect
75 Synergism Effect of Nisin Peptide in
Reducing Chemical Preservatives in Food Industry
Hamed
Haddad Kashani¹, Hosein Nikzad2, Saed Mobaseri*3, Elahe
Seyed Hoseini4
Anatomical Sciences Research Center,
Kashan University of Medical Sciences, Kashan, Iran¹.
Anatomical Sciences Research Center,
Kashan University of Medical Sciences, Kashan, Iran2.
Department
of Microbiology, Science Faculty, Azad University, Qom, Iran3.
Research
Center for Sciences and Biotechnology, Lavizan, Tehran. Iran4
Abstract: Due to
increasing interest to natural preservatives, many studies have been performed
in recent years. Nisin peptide as a natural preservative is very interesting
for the control of food pathogens and microorganisms of food spoilage. In this
study antibacterial activity of nisin and its synergism in decreasing
concentrations of common chemical preservatives like sodium nitrite and benzoic
acid against standard bacteria of Staphylococcus aureus and Lysteria
monocytogenes has been investigated. For this purpose, the MIC
(minimum inhibitory concentration) of nisin peptide, sodium nitrite and benzoic
acid were evaluated at different pH based on the dilution method. Results
showed that these compounds have antibacterial activity against Staphylococcus
aureus and Lysteria monocytogenes. MIC for nisin peptide,
sodium nitrite and benzoic acid in Staphylococcus aureus, was 350,
200, 25 (ppm) respectively. These values were 100, 200, 10 (ppm) in Lysteria
monocytogenes. It has been also shown that simultaneous use of nisin
with chemical preservatives sodium nitrite and benzoic acid reduced MIC of
these compounds against two strains of bacteria. Thissynergistic effect of
nisin could reduce the use of chemical preservatives in food industry.
[Hamed
Haddad Kashani, Hosein Nikzad, Saed Mobaseri, Elahe Seyed Hoseini. Synergism Effect of Nisin Peptide in
Reducing Chemical Preservatives in Food Industry. Life
Sci J 2012;9(1):496-501]. (ISSN: 1097-8135).http://www.lifesciencesite.com. 75
Keywords: Nisin, Cancer, Sodium nitrite, Benzoic acid, Bacteria. Full Text 75
