The Effect of Hum
The Effect of Human Bone Marrow Mesenchymal Stem Cells on Diabetic Heart Failure Rats.
Hany E. El Said1 , Hala M Gabr2 and Rasha I Ammar3
1Departnents of 1Physiology, 2Clinical Pathology and 3Pediatric Cardiology, Kasr Al-Aini Faculty of Medicine, Cairo University, Cairo, Egypt. [email protected]
Abstract: Aim: The purpose of this study was to investigate the effect of bone marrow mesenchymal stem cells (MSCs) on cardiovascular complications of type 1 diabetes mellitus (DM) in rats associated with heart failure. Material and Methods: BM-MSCs were derived from the human bone marrow . The MSCs were characterized morphologically and by RT-PCR for CD29 expression. They were then infused into rat tail vein which were they were made diabetic by IP injection of streptozotocin (STZ) and also we induce heart failure through injection of adramycin . The rats were divided into control, diabetic(D) , and diabetic and heart failure(D_HF) plus MSC groups where D-HF rats injected with human bone marrow derived stem cells(BM-MSC). Serum glucose, insulin, and fibrinogen were estimated in all groups . Physiological cardiovascular functions :Systolic and diastolic blood pressure, echocardiography were assessed . Homing of BM-MSCs in cardiac tissue and histological examination were done at the end of the experiment. Results: Diabetic rats which received MSCs showed significantly lower serum glucose and increased serum insulin levels compared with the D- HF group. Improvement of cardiovascular performance was also observed in the D-HF group compared with the D group. Homing of stem cells was detected in cardiac tissues of the BM-MSC group.Conclusions: Human bone marrow harbors cells that have the capacity to differentiate into functional insulin-producing cells capable of controlling blood glucose level in diabetic rats. Furthermore, MSC transplantation can improve cardiac function in diabetic rats associated with heart failure.
[Hany E. El Said , Hala M Gabr and Rasha I Ammar. The Effect of Human Bone Marrow Mesenchymal Stem Cells on Diabetic Heart Failure Rats. Life Sci J 201;10(1)3413-3425]. (ISSN: 1097-8135).http://www.lifesciencesite.com 3
Key words: Human bone marrow derived mesenchymal stem cells, diabetes, steptozotocin, heart failure , adramycin, rats.
