The Impact of Alc
The Impact of Alcohol on Sperm Parameters in diabetic mice
Authors: Pourentezari M1, Mangoli E1, Rahimipour M1, Talebi AR*1, 2, Anvari M1, 2
Address: Department of Biology & Anatomy1, Research & Clinical Center for Infertility2, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. *Corresponding author: [email protected]
Abstract: Diabetes mellitus (DM) is demonstrated one of the important stress to modern universal health. Reproductive disorder in diabetic males is fixed. Found that insulin-dependent diabetes is connected to reduce ejaculated semen and decreased vitality and motility of the spermatozoa. Alcohol consumption can have an adverse effect on sperm production. Alcohol abuse is considered as one of the problems associated with poor semen production and sperm quality. Objective: Given the importance of sperm in reproductive and Generation health other hand, diabetes and alcohol interaction aim of this study is survey the impact of alcohol on sperm parameters in mice with diabetes. Material and methods: 32 Adult male mice (10 weeks old, 35g) that they divided to 4 groups, mice of group 1 served as control fed on basal diet, group 2 received streptozotocin (STZ) (200 mg/kg, single dose, intraperitoneal) and basal diet, group 3 received alcohol (10 mg/kg, Water-soluble) and basal die and group 4 received streptozotocin and alcohol for 35 days. Finally, right tail of epididymis was cut in Ham’s F10. Released sperm were used to analyse number, motility, morphology (Pap-staining) and viability (eosin-Y staining) of the sperm.Result: In diabetes + alcohol mice, a significant decrease was found in sperm viability and sperm morphology compared to control, diabetes and alcohol mice, the morphology of sperm diabetes +alcohol more than other groups were beheaded. In diabetic and alcohol mice, a significant decrease was found in sperm motility and sperm count compared to control mice. Conclusion: Alcohol consumption in men with diabetes exacerbate damage to sperm, particular makes to increase the sperm beheaded
[ Pourentezari M, Mangoli E, Rahimipour M Talebi AR, Anvari M. The Impact of Alcohol on Sperm Parameters in diabetic mice. J Am Sci 2012;8(10):129-133]. (ISSN: 1545-1003).http://www.jofamericanscience.org 20
Key words: Mice, Sperm parameters, Diabetes, Alcohol. Full Text 20
