Attenuation of spe
Attenuation of specific
CTL responses by highly efficient transduction of the recombinant
adenovirus expressing His-tag-ICP47 fusion gene
Wang Peng 1, Zhang Zhenxiang 1*,
Kan Quancheng 2, Yu Zujiang 2, Li Ling 3,
Pan Xue 1 , He Hongjun 1, Feng Ting 1,
Li Xiangnan 1, Jiang Li-li 1 , Zhai
Guangyu 1 and Cui Guanglin 4
1. Nursing
College, Zhengzhou University, Zhengzhou, Henan 450052, China
2. Clinical
Pharmacology Base, First Affiliated Hospital, Zhengzhou University, Zhengzhou,
Henan 450052, China
3. Department
of Palliative Care and Hospice Care, the Ninth People’s Hospital of Zhengzhou,
Zhengzhou, Henan 450053, China 4. Department of Gastroenterology, Second
Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, China. [email protected]
Abstract: Hepatocyte transplantation (HT) has
been proposed as an alternative therapy to orthotopic liver
transplantation (OLT) for patients with acute liver failure and metabolic
disorders and hepatocytes are attractive targets for gene
therapy. Adenovirus vector is considered a safe and efficient way to introduce
foreign genes into several kinds of cells and is widely used in the various
fields of gene therapy. But the response of host immune systems against gene
products expressed by genetically modified cells is a major obstacle to
successful gene therapy. Major histocompatibility complex (MHC) class I antigen
presenting pathway is very important in acute allograft rejection and blocking
MHC I antigen expression is becoming a research hotspot of inducting
immune tolerance. Infected cell protein 47 (ICP47) expressed by herpes simplex
virus type 1 (HSV-1), inhibits MHC I antigen presentation pathway by
binding to host transporter associated with antigen presentation (TAP), and
thereby attenuates of specific cytotoxic T lymphocytes（CTL）responses by
virus-infected cells and evades the host immune clearance. This subject
was designed to construct a recombinant adenovirus expressing His-tag-ICP47
fusion protein to investigate further the role of ICP47 in the elimination of
transgene expression.
[Wang Peng, Zhang Zhenxiang, Kan Quancheng, Yu Zujiang, Li Ling, Pan Xue, He Hongjun, Feng Ting, Li Xiangnan, Jiang Li-li, Zhai Guangyu and Cui Guanglin. Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gene. Life Sci J2013;10(1):2069-2074] (ISSN:1097-8135). http://www.lifesciencesite.com.
Keywords: recombinant adenovirus; His-tag-ICP47 fusion gene; cytotoxic T lymphocytes.
