Characterization o
Characterization of a novel strain of the genus Actinopolyspora, an extremely
halophilic actinomycete isolated from Saudi Arabia
Manal J. Kiki 1, Idriss M. Al Turk1 2
1 Department of Biology, Faculty of science, King Abdulaziz University, Jeddah, Saudi Arabia
2 Department of Biology, Faculty of science, Taiba University, Al-Madiah Al-Munawwarh,
Saudi Arabia
Abstract: Actinomycetes have attracted a great attention due to their ability for production of various useful secondary metabolites. . In these work, an extremely halophilic filamentous actinomycetes was isolated from hyper saline soil sample collected from Jeddah region in the west of Saudi Arabia. This isolate can grow at high NaCl concentration up to 30% (w/v). Phylogenetic studies using16S rDNA gene sequence analysis was performed in order to specify the isolate. Results showed that morphological, physiological and biochemical characteristics of the isolate were matched to the genus Actinopolyspora. Basing on phylogenetic studies and searching the isolate against the EMBL public database, the isolate is proposed as novel strain of the genusActinopolyspora. The name Actinpolyspora saudiensis sp. nov. is proposed and the aim of the study was to describe the isolation, morphology, physiology and biochemical characteristics of this novel strain.
[Manal J. Kiki, Idriss M. Al Turk1. Characterization of a novel strain of the genus Actinopolyspora, an extremely halophilic actinomycete isolated from Saudi Arabia. Life Sci J 2013;10(1):603-608]. (ISSN: 1097-8135). http://www.lifesciencesite.com .7
Keywords: Actinopolyspora, halophilic actinomycete, saline soil, halophile, 16S rDNA sequencing.
