Prevalence and Mol
Prevalence and Molecular Detection of
Giardia and Cryptosporidium spp in Communities Consuming Different Drinking
Water Sources in Kohat, Khyber Pakhtunkhwa Pakistan
Sultan Ayaz1, Jameel A. Khader2, Liala
Rubab1, Riaz Ullah3*, Sanuallah Khan1, Raiz
Hussain4, Naser M. Abd E Islam2
1Department
of Zoology, Kohat University of science and technology Kohat 26000, Khyber
Pakhtunkhwa. Pakistan
2Arriyadh Community College, King Saud
University, Riyadh, Saudi Arabia
3Department of Chemistry, Sarhad University of Science
and Information Technology Peshawar KPK Pakistan
2Department
of Zoology, Garrison Cadet College Kohat, Khyber Pakhtunkhwa. Pakistan
Corresponding author; Dr
Riaz Ullah, [email protected]
Abstract: A
study was conducted to determine the presence of Giardia and cryptosporidium
parasites in drinking water sources of three different communities in kohat,
Khyber Pakhtunkhwa. A total of 150 water samples from three different sources
such as tap water (effluent), open well water, stream water (influent) were
examined by polymerase chain reaction (PCR). The average concentration of the
parasites in drinking water sources were ranged from 0.0 - 12% Giardia cyst and
cryptosporidium oocyst per litter in the concentrated pellet of the volume of
one litter. The DNA of the parasites was extracted through vivantis Tissue DNA
extraction kit, USA. A 163 bp Giardia spp and 256bp
cryptosporidium spp gene were amplified. overall prevalence was 12% (18/150),
amongst these Giardia Spp. and Cryptosporidium Spp.
were 5.33% (8/150) and 6.66% (10/150) in tap, well and spring water in Kohat,
Jauzara and Sheikhan Sherkot respectively. The presence of Giardia and
cryptosporidium spp in the examined water sources through PCR may needs the
purification and filtrations of the drinking water before its consumption in
kohat, Khyber Pakhtunkhwa.
[Sultan
Ayaz, Jameel
A. Khader, Liala Rubab, Riaz Ullah, Sanuallah Khan, Raiz
Hussain, Naser
M. Abd E Islam.Prevalence and Molecular Detection of Giardia and
Cryptosporidium spp in Communities Consuming Different Drinking Water Sources
in Kohat, Khyber Pakhtunkhwa Pakistan. Life
Sci J 2013;10(1):2708-2711] (ISSN:1097-8135). http://www.lifesciencesite.com.
Key words: PCR, Giardia, Cryptosporidium and DNA.
