Study on Vancomyci
Study on Vancomycin-Resistant Staphylococcus
aureus and Identification of VanA Gene in These Strains
Isolated from Tabriz Shuhada Hospital Using E-Test and PCR Methods
Sughra Sheikh Muneeri1, Haedeh
Mobaiyen2, Hamid Mirzaie3
1- M.S in Microbiology,
Zanjan Branch, Islamic Azad University, Zanjan, Iran
2- Department of
Microbiology, Faculty of Medical Science, Tabriz Branch, Islamic Azad
University, Tabriz, Iran
3- Department of Food
Hygiene, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad
University, Tabriz, Iran
Email: [email protected]
Abstract: Staphylococcus aureus is one of the main causes of hospital-acquired infections.
Vancomycin is widely used in treating infections created by this organism which
increases vancomycin-resistance and the spread of S. aureus strains
with intermediate vancomycin resistance in different parts of the world has
created major concerns about the clinical samples. Gathering data on the
situation of drug resistance - especially vancomycin-resistance of these
organisms not only is considered an effective measure to prevent hospital
infections but also as one of research priorities. In this study, the frequency
of vancomycin resistance and the presence of vanA gene are evaluated in S.
aureus strains isolated from Tabriz Shohada hospital with PCR.
73 strains of Staphylococcus aureus were isolated from
hospitalized patients in Tabriz Shohada Hospital during a period of eight
months. All of strains were identified through the routine methods. The
resistance to vancomycin was identified through agar disk diffusion method and
MIC of vancomycin was measured through E-test. Also, in order to identify vanA
genes, specific primers were applied. The frequency of intermediate
vancomycin-resistant strains using agar disk diffusion method and E-test are %
26.02 and % 14.43, respectively. The results of PCR indicate that 1.3% of
strains are identified as vancomycin-resistant. VanA- positive strains were far
more resistant against antibiotics than vanA- negative. During the present
study vanA gene was identified in only one strain of isolated S. aureus with
PCR. Separation of vanA gene in isolated strains rings the alarm for
authorities to control infection in the given hospital and other medical
centers in Tabriz.
[Sughra
Sheikh Muneeri, Haedeh Mobaiyen, Hamid Mirzaie. Study on Vancomycin-Resistant Staphylococcus
aureus and Identification of VanA Gene in These Strains Isolated from
Tabriz Shuhada Hospital Using E-Test and PCR Methods. Life Sci J 2013;10(1):748-752]. (ISSN:
1097-8135). http://www.lifesciencesite.com.
Key words: Staphylococcus aureus, Drug Resistance, vanA Gene, PCR, Vancomycin, E-test
