The Effect Of Asco
The Effect Of Ascorbic Acid
On Protection Of Hepatic Injury Consequence Effective Isomers Of Pentanedioic Acid In Rats
1Ramin
Amin Abad*, 2Naiyereh
Haerifar, 3Eilyad Issabeagloo
1- DVM, Faculty of Veterinary Medicine, Tabriz branch, Islamic Azad
University, Tabriz, Iran.
2- Department
of physiology, Medical Sciences Faculty, Tabriz branch, Islamic Azad
University, Tabriz, Iran.
3- Department
of Pharmacology, Medical Sciences Faculty, Tabriz
branch, Islamic Azad University, Tabriz, Iran.
ABSTRACT: Pentanedioic acid derivatives such as Methotrexate are anti-neoplastic agents and
are widely used in treatment of auto immune disorders and malignant diseases,
but their use is limited because of several side effects including
hepatotoxicity. As one of the important mechanisms involving in the
pathogenesis of methotrexate toxicity, some of anti-oxidant agents have been
used to reduce its side effect. Ascorbic acid (Vit. C) is an important
anti-oxidant drug and in this research we aimed to study vitamin C effect on
methotrexate induced hepatotoxicity. Thirty male Wistar rats were divided into
three groups (n=10). First group was control group, the second group was
Methotrexate group (represent pentanedioic acid derivatives) in which received,
and the third group was methotrexate plus vitamin C group whom received
methotrexate and vitamin C. On 6th day, the rats were
anesthetized and liver tissue removed for pathologic studying and plasma
isolated for measuring ALT and AST (P<0.05). Results showed that
vitamin C treatment decreased liver tissue injury and elevation of ALT and AST
after methotrexate administration. Regarding increasing use of methotrexate and
the results of this research Vitamin C may be helpful in suppressing
methotrexate induced hepatotoxicity and it is suggested to do this research on
other animal models and study the underlying mechanisms.
[Ramin
Amin Abad, Naiyereh haeri Far, Eilyad Issabeagloo. The Effect Of
Ascorbic Acid On Protection Of Hepatic Injury Consequence Effective Isomers Of
Pentanedioic Acid In Rats. Life Sci J 2013;10(7s):589-592].
(ISSN: 1097-8135).http://www.lifesciencesite.com.
94
Key
words: hepatotoxicity; ascorbic
acid; pentanedioic acid derivatives; Methotrexate; Rat.
