The Effect of Omeg
The Effect
of Omega- 3 Fatty Acids on the Age Related Changes in Submandibular Salivary
Glands of Albino Rats
Dalia EL-Baz
and Zainab A. Salem*
*Oral Biology
Department, Faculty of Oral and Dental Medicine, Cairo University, Egypt.
Abstract: Objective: The current study has been carried out to evaluate
histologically, immunohistochemistry and statistically the effect of daily
consumption of omega-3 on submandibular salivary gland of old aged rats.
Material & methods: Eighteen male albino rats were used in this
study. The rats were divided into three groups (6 rats/each): Group I: 4-6
months rats weighing 170-200 gm, represented the adult rats. Group II:
12-15 months rats weighing 250-300 gm, represented the old rats. Group
III: : 12-15 months rats weighing 250-300 gm, represented the old rats, and receiving
omega-3(60 mg/kg) once daily, for one month by intra- gastric intubation. All rats were sacrificed after 30 days. Soft
tissue specimens were obtained from submandibular salivary gland at the floor
of the mouth of the rats in all the studied groups. The sections were examined
histologically with H&E, immunohistochemically using tumor necrosis factor
alpha and the results were evaluated statistically. Results:Histopathological
changes were observed in group II including; decrease in the overall size of
the acini and granular convoluted tubules, an increase in the amount of fatty
tissue, fibrous tissue and inflammatory infiltration. In group III, the acini
and ducts appeared nearly normal and there was marked decrease in the
inflammatory infiltration. Immunohistochemical examination revealed moderate
expression of tumor necrosis factor alpha in group II, mild expression in group
III and weak expression in group I. Conclusion: Omega-3 had significant effects
in management of many age related changes of submandibular salivary gland.
[Dalia EL-Baz and Zainab A. Salem. The
Effect of Omega- 3 Fatty Acids on the Age Related Changes in Submandibular
Salivary Glands of Albino Rats. J Am Sci 2013;9(6):149-154].
(ISSN: 1545-1003).http://www.jofamericanscience.org. 17
Key words: Omega-3, Aging, Tumor necrosis factor alpha,
Submandibular salivary gland. Full Text 17
