Use of Fourier Tra
Use of Fourier Transform Infrared Spectroscopy to Study Cadmium-Induced Changes in Strongylocentrotus nudus gonad
ZhenlinWei
Biology Science Department, Dezhou University, Dezhou, Shandong, 253023, CHINA; [email protected]
Abstract: In this paper, the effects of 0.1 and 0.01 mM cadmium on Strongylocentrotus nudus gonad were studied by means of fourier transform infrared spectroscopy. The second derivative spectra and curve-fitting analysis revealed adverse effects of cadmium stress on the metabolism of lipids and proteins in gonad. For lipids, cadmium treatment shifted bands position to higher wavenumer and decreased bands area, especially those bands contributed from CH2 stretching vibration group, suggesting that the structure of lipids constituents were disordered and the contents decreased. Taken account to proteins, cadmium resulted in increasing fraction of β sheet structure and decreasing fraction of α helix, as well as declining protein contents. Compared with 0.01 mM treatment, the higher concentration showed more significant effects, such as bands position shifting to a larger extent and formation of 1692 cm-1 band within Amide I regions. The results demonstrated that FTIR spectroscopy is a promising tool for detection of cadmium induced changes in the context of molecular structure observation in urchin gonad.
[Weil ZL. Use of Fourier Transform Infrared Spectroscopy to Study Cadmium-Induced Changes inStrongylocentrotus nudus gonad. Life Sci J 2013;10(1):269-276] (ISSN:1097-8135).http://www.lifesciencesite.com. 41
Keywords: Cadmium stress; Strongylocentrotus nudus; Gonad; Fourier Transform Infrared Spectroscopy
