detection of New P
Detection of New Polychlorinated Biphenyl (PCB) Degraders Express Meta-cleavage Enzymes in PCB Contaminated Site
Hamdy A. Hassan
Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, Menoufia University, Sadat city, Egypt. [email protected]
Abstract: Oxidation ponds of Sadat city in Egypt had been polluted with polychlorinated Biphenyls (PCBs) compounds. The PCBs content in these ponds were detected with range from 212 to 320 mg PCBs kg-l. 27 bacterial isolates were isolated based on its capability to express meta-cleavage enzymes 2,3-dihydroxybiphenyl dioxygenase (DHBD) and catechol 2,3 dioxygenase (C23O) as a key enzyme of aerobic PCBs degradation. A partial 16S rRNA gene sequence revealed that the isolates belonged to the genusPseudomonas, Bacillus, Staphylococcus, Rhodococcus, Burkholderia, and Pandoraea. The 27 bacterial isolates had the capability to use monochlorinated biphenyl as the sole carbon source. Ten isolates were belonged to genus Pseudomonas, Burkholderia, Bacillus and Rhodococcus had also the capability to use dichlorinatedbiphenyl as the sole carbon source. Only two strains Burkholderia sp. HA-OP24 and Rhodococcus sp. HA-30 were capable of metabolizing the highly recalcitrant congener 2,4,5,2′,4′,5′-chlorobiphenyl. The new PCBs degraders harbor DHBD and C23O. These genes may act as functional genes in the metabolic pathway of the PCBs, which can be used as a suitable molecular marker for the in situ detection of microbial communities in PCBs contaminated sites. A good understanding of these novel strains, may aid not only in answering questions concerning the microbial degradation of polychlorinated biphenyls (PCBs) in natural systems but also to enhance the potential use of bioremediation by facilitating the detection and monitoring of the PCBs degrader with DHBD and C2,3O in the contaminated sites.
[Hamdy A. Hassan. Detection of New Polychlorinated Biphenyl (PCB) Degraders Express Meta-cleavage Enzymes in PCB Contaminated Site. Life Sci J 2013;10(1):1123-1132] (ISSN:1097-8135).http://www.lifesciencesite.com.
Keywords: Bacterial community / meta-cleavage enzymes / Polychlorinated Biphenyls (PCBs)
